Last interglacial vegetation and climate history from the Portuguese coast          下载免费PDF全文

T. A. MINCKLEY  J. A. HAWS  M. M. BENEDETTI  S. C. BREWER  S. L. FORMAN 《第四纪科学杂志》2015,30(1):59-69

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条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选     

董道英  孔凡娜  崔正彩  孙斌 《海洋与湖沼》2019,50(6):1271-1280

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。  相似文献   

The Blake Event recorded near the Eemian type locality – A diachronic onset of the Eemian in Europe     

《Quaternary Geochronology》2015

Multidisciplinary analysis including paleomagnetic, sedimentologic, sea-level change, luminescence dating and palynologic research was performed on a 25 m long orientated core taken at Rutten, close to Eemian key localities in the Netherlands. The main goal of our research was to test a possible delayed onset of temperate conditions in this region compared to Southern Europe, occurring within the Last Interglacial. The sediments revealed the presence of the paleomagnetic Blake Event in ca. 10 m of lower-deltaic floodbasin sediments that contain a pollen record covering the Eemian. The position of the Blake Event in relation to the pollen stratigraphy concurs with the earlier studied Neumark Nord 2 site. Paleomagnetic correlation to core MD95-2042 off SW Iberia indicates ca. 5 kyr diachroneity between the pollen-based onset of temperate interglacial conditions between northern and southern Europe. The onset of the Eemian in north-western and central Europe (ca. 121.0 ka) post-dates the Marine Isotope Stage 6/5e transition by ca. 10 kyr. In addition, the Rutten data provide evidence for a relatively long duration of the Blake Event of at least 8 kyr. The late onset of the temperate conditions that define the base of the Eemian, imply that NW Europe with the Eemian type area is not the most suited region to define the beginning of the Last Interglacial and Late Pleistocene for global chronostratigraphic use.  相似文献   

Remote estimation of terrestrial evapotranspiration by Landsat 5 TM and the SEBAL model in cold and high‐altitude regions: a case study of the upper reach of the Shule River Basin,China          下载免费PDF全文

Yaping Chang  Yongjian Ding  Qiudong Zhao  Shiqiang Zhang 《水文研究》2017,31(3):514-524

Evapotranspiration (ET) is an important expenditure in water and energy balances, especially on cold and high‐altitude land surfaces. Daily ET of the upper reach of the Shule River Basin was estimated using Landsat 5 TM data and the Surface Energy Balance Algorithm for Land (SEBAL) model. Based on observations made at the Suli station, the algorithms of land surface temperature and soil heat flux in SEBAL were modified. Land surface temperature was retrieved and compared with ground truth via three methods: the radiative transfer equation method, the mono‐window algorithm, and the single‐channel method. We selected the best of these methods, mono‐window algorithm, for estimating ET. The average error of daily ET estimated by the modified SEBAL model and measured by the eddy covariance system was 16.4%, with a root‐mean‐square error of 0.52 mm d^?1. The estimated ET means were 3.09, 2.48, and 1.48 mm d^?1 on June 9 (DOY 160), June 25 (DOY 176), and July 27 (DOY 208) of the year 2010, respectively. The average estimated ET on the glacier surface of all days was more than 3 mm d^?1, a measurement that is difficult to capture in‐situ and has rarely been reported. This study will improve the understanding of water balance in cold, high‐altitude regions. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

CMIP5全球气候模式对中国上空空气静稳日数模拟能力评估          下载免费PDF全文

吴婕  徐影  周波涛 《地球物理学报》2017,60(4):1293-1304

空气静稳日数变化与污染物浓度变化密切相关,评估气候模式对空气静稳日数的模拟能力是进行未来预估的基础.本文利用15个CMIP5(Coupled Model Intercomparison Project phase 5)全球模式的模拟结果与观测数据,分别计算了1961-2005年逐年中国上空空气静稳日数,并利用统计方法分析了中国上空空气静稳日数的标准差、相对均方根误差、区域平均的时间序列、趋势分布和EOF(Empirical Orthogonal Function)主要模态变化特征,评估了CMIP5模式对中国上空空气静稳日数的模拟能力.结果表明:多模式集合平均结果可以模拟出空气静稳日数由沿海向内陆逐渐增加的分布特征,单个模式对空气静稳日数空间分布的模拟能力相差较大.多模式集合平均可以较好地再现夏、冬季的空气静稳日数.15个模式中,CanESM2和:IPSL-CM5B-LR对中国大部分区域的模拟效果较好,多模式集合平均的模拟能力优于单个模式.与观测相比,多模式集合平均的1961-2005年空气静稳日数年际变化波动较小,多数区域的多模式集合平均的空气静稳日数高于观测值.对于逐年的冬季空气静稳日数,大多数区域的多模式集合平均存在高估.在中国东部和新疆大部,多模式集合平均可以较好的模拟出空气静稳日数变化趋势的空间分布特征,但是数值偏小.多模式集合平均也能较好的模拟出空气静稳日数的EOF1和EOF2特征向量分布型,但对前三个EOF的时间系数序列模拟能力差.  相似文献   

Time‐series high‐resolution spectroscopy and photometry of ε Aurigae from 2006–2013: Another brick in the wall     

K. G. Strassmeier  M. Weber  T. Granzer  L. Schanne  J. Bartus  I. Ilyin 《Astronomische Nachrichten》2014,335(9):904-934

We present continuous and time‐resolved R = 55 000 optical échelle spectroscopy of ε Aurigae from 2006–2013. Data were taken with the STELLA Echelle Spectrograph of the robotic STELLA facility at the Observatorio del Teide in Tenerife. Contemporaneous photometry with the Automatic Photoelectric Telescopes at Fairborn Observatory in Arizona is presented for the years 1996–2013. Spectroscopic observations started three years prior to the photometric eclipse and are still ongoing. A total of 474 high‐resolution échelle spectra are analyzed and made available in this paper. We identify 368 absorption lines of which 161 lines show the characteristic sharp disk lines during eclipse. Another 207 spectral lines appeared nearly unaffected by the eclipse. From spectrum synthesis, we obtained the supergiant atmospheric parameters T_eff = 7395 ± 70 K, log g ≈ 1, and [Fe/H] = +0.02 ± 0.2 with ξ_t = 9 km s^–1, ζ_RT = 13 km s^–1, and v sin i = 28 ± 3 km s^–1. The residual average line broadening expressed in km s^–1 varies with a period of 62.6 ± 0.7 d, in particular at egress and after the eclipse. Two‐dimensional line‐profile periodograms show several periods, the strongest with ≈110 d evident in optically thin lines as well as in the Balmer lines. Center‐of‐intensity weighted radial velocities of individual spectral lines also show the 110‐d period but, again, additional shorter and longer periods are evident and are different in the Balmer lines. The two main spectroscopic Hα periods, ≈ 116 d from the line core and ≈ 150 d from the center‐of‐intensity radial velocities, appear at 102 d and 139 d in the photometry. The Hβ and Johnson V I photometry on the other hand shows two well‐defined and phase‐coherent periods of 77 d and 132 d. We conclude that Hα is contaminated by changes in the circumstellar environment while the Hβ and V I photometry stems predominantly from the non radial pulsations of the F0 supergiant. We isolate the disk‐rotation profile from 61 absorption lines and found that low disk eccentricity generally relates to low disk rotational velocity (but not always) while high disk eccentricity always relates to high velocity. There is also the general trend that the disk‐absorption in spectral lines with higher excitation potential comes from disk regions with higher eccentricity and thus also with higher rotational velocity. The dependency on transition probability is more complex and shows a bi‐modal trend. The outskirts of the disk is distributed asymmetrically around the disk and appears to have been built up mostly in a tail along the orbit behind the secondary. Our data show that this tail continues to eclipse the F0 Iab primary star even two years after the end of the photometric eclipse. High‐resolution spectra were also taken of the other, bona‐fide, visual‐binary components of ε Aur (ADS 3605BCDE). Only the C‐component, a K3‐4‐giant, appears at the same distance than ε Aur but its radial velocity is in disagreement with a bound orbit. The other components are a nearby (≈ 7 pc) cool DA white dwarf, a G8 dwarf, and a B9 supergiant, and not related to ε Aur. The cool white dwarf shows strong DIB lines that suggest the existence of a debris disk around this star. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

单侧控制条件下SPOT5 HRG影像的定向试验     

王铁军  孙洪双  马治  郑福海 《测绘软科学研究》2010,(6):62-65

高分辨率卫星影像已经成为获取地理信息的重要数据源,而其定向是一项关键技术。本文利用单侧不同数量及分布的控制点对SPOT5 HRG影像进行了定向试验,探讨了在单侧控制条件下SPOT5 HRG影像的定向精度情况,为地面控制点获取较困难地区的影像定向提供了一条参考途径。  相似文献   

Integrating downscaled CMIP5 data with a physically based hydrologic model to estimate potential climate change impacts on streamflow processes in a mixed‐use watershed          下载免费PDF全文

Michael G. Sunde  Hong S. He  Jason A. Hubbart  Michael A. Urban 《水文研究》2017,31(9):1790-1803

Climatic changes have altered surface water regimes worldwide, and climate projections suggest that such alterations will continue. To inform management decisions, climate projections must be paired with hydrologic models to develop quantitative estimates of watershed scale water regime changes. Such modeling approaches often involve downscaling climate model outputs, which are generally presented at coarse spatial scales. In this study, Coupled Model Intercomparison Project Phase 5 climate model projections were analyzed to determine models representing severe and conservative climate scenarios for the study watershed. Based on temperature and precipitation projections, output from GFDL‐ESM2G (representative concentration pathway 2.6) and MIROC‐ESM (representative concentration pathway 8.5) were selected to represent conservative (Δ_C) and severe (Δ_S) change scenarios, respectively. Climate data were used as forcing for the soil and water assessment tool to analyze the potential effects of climate change on hydrologic processes in a mixed‐use watershed in central Missouri, USA. Results showed annual streamflow decreases ranging from ?5.9% to ?26.8% and evapotranspiration (ET) increases ranging from +7.2% to +19.4%. During the mid‐21st century, sizeable decreases to summer streamflow were observed under both scenarios, along with large increases of fall, spring, and summer ET under Δ_S. During the late 21st century period, large decreases of summer streamflow under both scenarios, and large increases to spring (Δ_S), fall (Δ_S) and summer (Δ_C) ET were observed. This study demonstrated the sensitivity of a Midwestern watershed to future climatic changes utilizing projections from Coupled Model Intercomparison Project Phase 5 models and presented an approach that used multiple climate model outputs to characterize potential watershed scale climate impacts.  相似文献   

雨生红球藻中crtO基因存在3个5’上游侧翼序列     

高政权  孟春晓  叶乃好 《海洋通报(英文版)》2010,12(1):59-64

在本研究中，我们首次通过基因组上游第一次步移克隆到了雨生红球藻中crtO基因的3个不同大小的5’上游侧翼序列1．1kb，1．9kb和2．2kb。利用生物信息学方法分别预测并比较了这3个不同大小的5’上游侧翼序列，结果发现，三者具有与高等植物相类似的顺式作用元件，如脱落酸反应元件（ABRE）、干燥或低温反应元件（DRE／C-repeat）、几种光反应元件（G1-box,GAG-motif，l-boxand ATC—motif）、热激反应元件（HSE）、机械伤害反应元件（WUN-motif）、生长素反应元件（TGA-element）、茉莉酸甲酯反应元件（TGACG-element）和反式作用因子MYB蛋白的结合位点（MBSandMRE）等等，但是三者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中crtO基因调控方式的多样化。  相似文献   

Seasonal evaluation of rainfall estimation by four cumulus parameterization schemes and their sensitivity analysis     

Asnor Muizan Ishak  Michaela Bray  Renji Remesan  Dawei Han 《水文研究》2012,26(7):1062-1078

Sustainable water resources management require scientifically sound information on precipitation, as it plays a key role in hydrological responses in a catchment. In recent years, mesoscale weather models in conjunction with hydrological models have gained great attention as they can provide high‐resolution downscaled weather variables. Many cumulus parameterization schemes (CPSs) have been developed and incorporated into three‐dimensional Pennsylvania State University/National Center for Atmospheric Research (PSU/NCAR) mesoscale model 5 (MM5). This study has performed a comprehensive evaluation of four CPSs (the Anthes–Kuo, Grell, Betts–Miller and Kain–Fritsch93 schemes) to identify how their inclusion influences the mesoscale model's precipitation estimation capabilities. The study has also compared these four CPSs in terms of variability in rainfall estimation at various horizontal and vertical levels. For this purpose, the MM5 was nested down to resolution of 81 km for Domain 1 (domain span 21 × 81 km) and 3 km for Domain 4 (domain span 16 × 3 km), respectively, with vertical resolutions at 23, 40 and 53 vertical levels. The study was carried out at the Brue catchment in Southwest England using both the ERA‐40 reanalysis data and the land‐based observation data. The performances of four CPs were evaluated in terms of their ability to simulate the amount of cumulative rainfall in 4 months in 1995 representing the four seasonal months, namely, January (winter), March (spring), July (summer) and October (autumn). It is observed that the Anthes–Kuo scheme has produced inferior precipitation values during spring and autumn seasons while simulations during winter and summer were consistently good. The Betts–Miller scheme has produced some reasonable results, particularly at the small‐scale domain (3 km grid size) during winter and summer. The KF2 scheme was the best scheme for the larger‐scale (81 km grid size) domain during winter season at both 23 and 53 vertical levels. This scheme tended to underestimate rainfall for other seasons including the small‐scale domain (3 km grid size) in the mesoscale. The Grell scheme was the best scheme in simulating rainfall rates, and was found to be superior to other three schemes with consistently better results in all four seasons and in different domain scales. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献